Epitope Characterization is Critical Attributes for Early Identification of Leads with Desired MOA and Sought Functionality
Early identification of drug lead candidates with desired kinetics properties, targeting sought epitopes and expressing at good yields are critical quality attributes for avoiding later-phase failures caused by selecting non-ideal bioactive compounds.
Octet® BLI cross-competition epitope binning assays rely on discriminating proteins bind the same antigen but not the same epitope. The epitope binning matrix enable to gather binders that recognize similar epitopic regions into same bin. Because binders in different bins bind to distinct epitopes, epitope binning studies increase the likelihood of choosing lead candidates with non-redundant biological activities.
Cross-competition epitope binning assays have successfully been used in a plethora of applications to select binders targeting specific epitope (e.g generation of biosimilars compounds) or on the contrary selecting binders targeting new epitopes (e.g MOA differentiation).
During primary screening campaign, early BLI epitope targeting assays can be implemented to ensure the selection of hits according to a wide epitopic diversity.
All these information may generate data to establish IP and demonstrate differentiation from other biopharma.
Insight of BLI Cross-Competition Epitope Binning and Epitope Targeting Assays
BLI cross-competition epitope binning assays can accommodate multiple assay orientations that enable the analysis purified samples or samples from crude supernatant (e.g hybridoma, cell or bacteria culture). The set-up are versatile enough to enable the combination of different molecular formats of binding pairs (e.g monospecific Ab versus bispecific Ab, IgG versus ScFv, ssDNA versus protein…).
The system offers an exceptional reproducibility and its throughput is ideal for evaluating large antibody data sets (matrix of 100 pair combinations analyzed over a single day).
Insight of BLI Epitope Binning Pair Combination Matrix
BindingSolution offers versatile BLI binding measurement service platform to provide detailed insights into your product’s biological interactions.
BindingSolution’s expertise can help you to streamline and empower your drug discovery and development processes.
